A Novel Phenanthrene and An Undescribed Alkaloid from the Roots of Stephania tetrandra

A novel phenanthrene compound, diphenanthrin (1), and an undescribed alkaloid compound, N- chloromethylstephenanthrine (2), together with two known compounds (3-4) were isolated from the EtOH extract of the roots of Stephania tetrandra. Their structures were elucidated by 1D and 2D NMR, mass spectroscopy and comparison of NMR data with those of known compounds. In anti-inflammatory assay, compounds 3 and 4 showed the inhibitory effects on NO production, with IC50 values of 41.51 +/- 0.20 and 23.87 +/- 2.12 mu M.

Automated summary

A novel phenanthrene compound diphenanthrin (1) and an undescribed alkaloid compound N-chloromethylstephenanthrine (2), along with two known compounds (3-4), were isolated from the roots of Stephania tetrandra. Their structures were determined using 1D and 2D NMR, mass spectroscopy, and by comparing NMR data with those of known compounds. Compounds 3 and 4 exhibited inhibitory effects on NO production in an anti-inflammatory assay, with IC50 values of 41.51 +/- 0.20 and 23.87 +/- 2.12 μM.