A nanoplatform self-assembled by coordination delivers siRNA for lung cancer therapy

The high incidence and mortality of lung cancer have present threaten in front of people all over the world. Researches in clinical trials find that mutations of some genes influence progress of lung cancer directly or indirectly, therefore, some kinds of molecular inhibitors benefit patients in clinical therapy, which helpfully prolong survival time of patients and show great potential in lung cancer therapy. siRNA is a kind of nucleic acid molecules which can silence targeted gene translation through binding to mRNA completely to cure diseases. The delivery of siRNA for cancer therapy mostly can be classified into loading through electrostatic interaction, physical surrounding, and chemically modification. Yet delivering siRNA by coordination has not been reported. This study unprecedently utilizes the coordination between siRNA and Fe2+ to form a self-assembly structure in which doxorobicin (DOX) and human serum albumin (HSA) were used to stabilize the whole nanoplatform followed polyethylenimine (PEI) coating. Through the heat incubation strategy, highly loading efficiency for siRNA and DOX was achieved. This nanoplatform with stability and sustain release of drugs exhibited good lysosome escape, gene silencing effect and cytotoxicity which provided new horizon for co-delivery of siRNA and other molecular or protein drugs.

Automated summary

The high incidence and mortality of lung cancer pose a global threat. Clinical trials have shown that mutations in certain genes directly or indirectly affect the progress of lung cancer. As a result, molecular inhibitors have been found to benefit patients in clinical therapy, helping to prolong survival time and showing great potential in lung cancer treatment. siRNA, a nucleic acid molecule, can silence targeted gene translation by binding to mRNA. In this study, siRNA is delivered by coordination with Fe2+ to form a self-assembly structure, with doxorubicin (DOX) and human serum albumin (HSA) used for stabilization. The nanoplatform exhibited good lysosome escape, gene silencing effect, and cytotoxicity, providing new possibilities for co-delivery of siRNA and other molecular or protein drugs.