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NATL ACAD SCIENCES
DOI: 10.1073/pnas.2218163120
关键词
commensalism; mycology; adaptation; fitness; karyotype
Aneuploidy, a common phenomenon in fungi, can alter gene expression and promote adaptation to environmental cues. In a study on Candida albicans, a fungal pathogen, researchers found that a strain with a trisomy of chromosome 7 exhibited increased fitness during colonization and infection. The presence of this trisomy resulted in decreased filamentation, and the NRG1 gene on chromosome 7 played a role in inhibiting filamentation. These findings highlight how aneuploidy enables C. albicans to adapt to its host through gene dosage-dependent regulation of morphology.
Aneuploidy is a frequent occurrence in fungal species where it can alter gene expres-sion and promote adaptation to a variety of environmental cues. Multiple forms of aneuploidy have been observed in the opportunistic fungal pathogen Candida albi-cans, which is a common component of the human gut mycobiome but can escape this niche and cause life-threatening systemic disease. Using a barcode sequencing (Bar-seq) approach, we evaluated a set of diploid C. albicans strains and found that a strain carrying a third copy of chromosome (Chr) 7 was associated with increased fitness during both gastrointestinal (GI) colonization and systemic infection. Our analysis revealed that the presence of a Chr 7 trisomy resulted in decreased filamen-tation, both in vitro and during GI colonization, relative to isogenic euploid controls. A target gene approach demonstrated that NRG1, encoding a negative regulator of filamentation located on Chr 7, contributes to increased fitness of the aneuploid strain due to inhibition of filamentation in a gene dosage-dependent fashion. Together, these experiments establish how aneuploidy enables the reversible adaptation of C. albicans to its host via gene dosage-dependent regulation of morphology.
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