期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2303392120
关键词
phosphatidylserine; phagocytosis; da neurons; epidermal cells; Orion
This study reveals that the chemokine-like protein Orion in Drosophila can bind to phosphatidylserine (PS) exposed on the surface of degenerating neurons and mediate phagocytosis. Orion is also involved in facilitating the interaction between PS and the engulfment receptor Draper (Drpr). The dosage of Orion is a crucial determinant of the sensitivity of phagocytes to PS exposed on neurons.
Phagocytic clearance of degenerating neurons is triggered by eat -me signals exposed on the neuronal surface. The conserved neuronal eat -me signal phosphatidylserine (PS) and the engulfment receptor Draper (Drpr) mediate phagocytosis of degenerating neurons in Drosophila. However, how PS is recognized by Drpr-expressing phagocytes in vivo remains poorly understood. Using multiple models of dendrite degeneration, we show that the Drosophila chemokine-like protein Orion can bind to PS and is responsible for detecting PS exposure on neurons; it is supplied cell- non-autonomously to coat PS-exposing dendrites and to mediate interactions between PS and Drpr, thus enabling phagocytosis. As a result, the accumulation of Orion on neurons and on phagocytes produces opposite outcomes by potentiating and suppressing phagocytosis, respectively. Moreover, the Orion dosage is a key determinant of the sensitivity of phagocytes to PS exposed on neurons. Lastly, mutagenesis analyses show that the sequence motifs shared between Orion and human immunomodulatory proteins are important for Orion function. Thus, our results uncover a missing link in PS-mediated phagocytosis in Drosophila and imply conserved mechanisms of phagocytosis of neurons.
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