期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2302019120
关键词
aging; lymphopoiesis; hematopoietic stem cells
Mammalian aging is associated with defects in hematopoiesis, particularly in the development of T and B lymphocytes. This impairment is believed to be caused by the accumulation of hematopoietic stem cells (HSCs) with myeloid bias in the bone marrow. However, research using genetic labeling and tracing techniques showed that the HSC population in old mice had reduced differentiation into all lineages, including lymphoid cells. The partial decoupling of old HSCs from hematopoiesis, rather than myeloid bias, was found to be the primary cause of the selective impairment of lymphopoiesis in older mice.
Mammalian aging is associated with multiple defects of hematopoiesis, most prominently with the impaired development of T and B lymphocytes. This defect is thought to originate in hematopoietic stem cells (HSCs) of the bone marrow, specifically due to the age-dependent accumulation of HSCs with preferential megakaryocytic and/ or myeloid potential (myeloid bias). Here, we tested this notion using inducible genetic labeling and tracing of HSCs in unmanipulated animals. We found that the endogenous HSC population in old mice shows reduced differentiation into all lineages including lymphoid, myeloid, and megakaryocytic. Single-cell RNA sequencing and immunophenotyping (CITE-Seq) showed that HSC progeny in old animals comprised balanced lineage spectrum including lymphoid progenitors. Lineage tracing using the aging-induced HSC marker Aldh1a1 confirmed the low contribution of old HSCs across all lineages. Competitive transplantations of total bone marrow cells with genetically marked HSCs revealed that the contribution of old HSCs was reduced, but compensated by other donor cells in myeloid cells but not in lymphocytes. Thus, the HSC population in old animals becomes globally decoupled from hematopoiesis, which cannot be compensated in lymphoid lineages. We propose that this partially compensated decoupling, rather than myeloid bias, is the primary cause of the selective impairment of lymphopoiesis in older mice.
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