期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2301215120
关键词
metabolomics; isotope tracing; stress; catecholamine; in vivo
The method of blood sample collection has significant effects on plasma metabolomics and stable isotope tracing in mice. Stress and sampling site both contribute to differences in the circulating metabolome. Lactate is the most important metabolite in unstressed mice metabolism and is produced in response to acute stress. Noninvasive arterial sampling can avoid these artifacts.
Plasma metabolite concentrations and labeling enrichments are common measures of organismal metabolism. In mice, blood is often collected by tail snip sampling. Here, we systematically examined the effect of such sampling, relative to gold-standard sampling from an in-dwelling arterial catheter, on plasma metabolomics and stable isotope trac-ing. We find marked differences between the arterial and tail circulating metabolome, which arise from two major factors: handling stress and sampling site, whose effects were deconvoluted by taking a second arterial sample immediately after tail snip. Pyruvate and lactate were the most stress-sensitive plasma metabolites, rising similar to 14 and similar to 5-fold. Both acute handling stress and adrenergic agonists induce extensive, immediate production of lactate, and modest production of many other circulating metabolites, and we provide a reference set of mouse circulatory turnover fluxes with noninvasive arterial sampling to avoid such artifacts. Even in the absence of stress, lactate remains the highest flux circu-lating metabolite on a molar basis, and most glucose flux into the TCA cycle in fasted mice flows through circulating lactate. Thus, lactate is both a central player in unstressed mammalian metabolism and strongly produced in response to acute stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据