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出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2216310120
关键词
IGF; insulin signaling; serum-and glucocorticoid-regulated kinase 1; mitochondrial membrane potential; reactive oxygen species; F1Fo-ATP synthase
We investigated the reactivation of quiescent cells through a zebrafish model and observed an increase in mitochondrial membrane potential in the reactivated cells. Genetic and pharmacological perturbations revealed that elevated mitochondrial metabolism and ATP synthesis are crucial for cell reactivation. Further analysis showed that the elevated mitochondrial metabolism leads to increased mitochondrial ROS levels, which induce the expression of Sgk1 in the mitochondria. Sgk1, in turn, promotes S phase entry in human breast cancer cells by coordinating mitochondrial activity with ATP synthesis through phosphorylating F1Fo-ATP synthase.
Many types of differentiated cells can reenter the cell cycle upon injury or stress. The underlying mechanisms are still poorly understood. Here, we investigated how quiescent cells are reactivated using a zebrafish model, in which a population of differentiated epithelial cells are reactivated under a physiological context. A robust and sustained increase in mitochondrial membrane potential was observed in the reactivated cells. Genetic and pharmacological perturbations show that elevated mitochondrial metabolism and ATP synthesis are critical for cell reactivation. Further analyses showed that elevated mitochondrial metabolism increases mitochondrial ROS levels, which induces Sgk1 expression in the mitochondria. Genetic deletion and inhibition of Sgk1 in zebrafish abolished epithelial cell reactivation. Similarly, ROS-dependent mitochondrial expression of SGK1 promotes S phase entry in human breast cancer cells. Mechanistically, SGK1 coordinates mitochondrial activity with ATP synthesis by phosphorylating F1Fo-ATP synthase. These findings suggest a conserved intramitochondrial signaling loop regulating epithelial cell renewal.
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