期刊
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES
卷 99, 期 3, 页码 75-101出版社
JAPAN ACAD
DOI: 10.2183/pjab.99.006
关键词
tyrosine phenol-lyase; L-DOPA; Parkinson?s disease; Escherichia intermedia; Erwinia herbicola; TyrR
This article discusses a simple enzymatic synthesis method using bacterial L-tyrosine phenol-lyase (Tpl) for the production of L-DOPA, a treatment for Parkinson's disease. The research covers screening of bacterial strains, culture conditions, properties and reaction mechanism of the enzyme, as well as the reaction conditions for L-DOPA production. Additionally, the article explores molecular breeding of Tpl-over-producing strains through mutations in the DNA binding protein TyrR.
L-DOPA is an amino acid that is used as a treatment for Parkinson's disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-over-producing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据