期刊
JOURNAL OF VIROLOGICAL METHODS
卷 234, 期 -, 页码 7-15出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2016.02.015
关键词
Equine arteritis virus; Equine viral arteritis; Equine semen; Abortion; Real-time RT-PCR; Insulated isothermal RT-PCR
资金
- Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2013-68004-20360]
- NIFA [688708, 2013-68004-20360] Funding Source: Federal RePORTER
Equine arteritis virus (EAV) is the causative agent of equine viral arteritis (EVA), a respiratory and reproductive disease of horses. Most importantly, EAV induces abortion in pregnant mares and can establish persistent infection in up to 10-70% of the infected stallions, which will continue to shed the virus in their semen. The objective of this study was to develop and evaluate a reverse transcription insulated isothermal polymerase chain reaction (RT-iiPCR) for the detection of EAV in semen and tissue samples. The newly developed assay had a limit of detection of 10 RNA copies and a 10-fold higher sensitivity than a previously described real-time RT-PCR (RT-qPCR). Evaluation of 125 semen samples revealed a sensitivity and specificity of 98.46% and 100.00%, respectively for the RT-qPCR assay, and 100.00% and 98.33%, respectively for the RT-iiPCR assay. Both assays had the same accuracy (99.2%, k = 0.98) compared to virus isolation. Corresponding values derived from testing various tissue samples (n = 122) collected from aborted fetuses, foals, and EAV carrier stallions are as follows: relative sensitivity, specificity, and accuracy of 88.14%, 96.83%, and 92.62% (k = 0.85), respectively for the RT-gPCR assay, and 98.31%, 92.06%, and 95.08% (k = 0.90), respectively for the RT-iiPCR assay. These results indicate that RT-iiPCR is a sensitive, specific, and a robust test enabling detection of EAV in semen and tissue samples with very considerable accuracy. Even though the RT-qPCR assay showed a sensitivity and specificity equal to virus isolation for semen samples, its diagnostic performance was somewhat limited for tissue samples. Thus, this new RT-iiPCR could be considered as an alternative tool in the implementation of EAV control and prevention strategies. (C) 2016 Elsevier B.V. All rights reserved.
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