期刊
JOURNAL OF VIROLOGICAL METHODS
卷 228, 期 -, 页码 151-157出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2015.11.020
关键词
PCR; Respiratory pathogens; Comparative studies; Analytical techniques; Microfluidics
资金
- Centers for Disease Control and Prevention [U60HM000803]
- National Center for Immunization and Respiratory Diseases (IP)
- Office of Surveillance, Epidemiology and Laboratory Services (OSELS) (OE)
- National Center for HIV, Viral Hepatitis, STDs and TB Prevention (PS)
- National Center for Zoonotic, Vector-Borne and Enteric Diseases (CK)
- National Center for Environmental Health (NCEH)
- Coordinating Office for Terrorism Preparedness and Emergency Response (CTPER)
In this study, a multicenter evaluation of the Life Technologies TaqMan (R) Array Card (TAC) with 21 custom viral and bacterial respiratory assays was performed on the Applied Biosystems ViiA (TM) 7 Real-Time PCR System. The goal of the study was to demonstrate the analytical performance of this platform when compared to identical individual pathogen specific laboratory developed tests (LDTs) designed at the Centers for Disease Control and Prevention (CDC), equivalent LDTs provided by state public health laboratories, or to three different commercial multi-respiratory panels. CDC and Association of Public Health Laboratories (APHL) LDTs had similar analytical sensitivities for viral pathogens, while several of the bacterial pathogen APHL LDTs demonstrated sensitivities one log higher than the corresponding CDC LDT. When compared to CDC LDTs, TAC assays were generally one to two logs less sensitive depending on the site performing the analysis. Finally, TAC assays were generally more sensitive than their counterparts in three different commercial multi-respiratory panels. TAC technology allows users to spot customized assays and design TAC layout, simplify assay setup, conserve specimen, dramatically reduce contamination potential, and as demonstrated in this study, analyze multiple samples in parallel with good reproducibility between instruments and operators. (C) 2015 Elsevier B.V. All rights reserved.
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