期刊
JOURNAL OF VIROLOGICAL METHODS
卷 237, 期 -, 页码 132-137出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2016.09.007
关键词
HIV-1; Diagnosis; Point-of-care; RNA; Nucleic acid amplification
资金
- University of Washington Center for AIDS Research (CFAR)
- NIH - NIH (NIAID) [AI027757]
- NIH (NCI)
- NIH (NIMH)
- NIH (NIDA)
- NIH (NICHD)
- NIH (NHLBI)
- NIH (NIA)
- NIH (NIGMS)
- NIH (NIDDK)
- NIH - NIBIB [R01EB012641]
Early and accurate diagnosis of HIV is key for the reduction of transmission and initiation of patient care. The availability of a rapid nucleic acid test (NAT) for use at the point-of-care (POC) will fill a gap in HIV diagnostics, improving the diagnosis of acute infection and HIV in infants born to infected mothers. In this study, we evaluated the performance of non-instrumented nucleic acid amplification, single-use disposable (NINA-SUD) devices for the detection of HIV-1 in whole blood using reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) with lyophilized reagents. The NINA-SUD heating device harnesses the heat from an exothermic chemical reaction initiated by the addition of saline to magnesium iron powder. Reproducibility was demonstrated between NINA-SUD units and comparable, if not superior, performance for detecting clinical specimens was observed as compared to the thermal cycler. The stability of the lyophilized HIV-1 RT-LAMP reagents was also demonstrated following storage at -20, 4, 25, and 30 degrees C for up to one month. The single-use, disposable NAT minimizes hands-on time and has the potential to facilitate HIV-1 testing in resource-limited settings or at the POC. Published by Elsevier B.V.
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