BAG6-A from Fragaria viridis pollen modulates gametophyte development in diploid strawberry

Male and female gametophyte development processes are essential steps in the life cycles of all land plants. Here, we characterized a gene, FviBAG6-A, screened from the Fragaria viridis (2 n = 2x=14) pollen cDNA library and physically interacted with S-RNase. Ubiquitinated of Sa-RNase might be determined by the interaction of FviBAG6-A in the ubiquitin-proteasome system during fertilization. We found that overexpression of FviBAG6-A in Arabidopsis caused shorter silique length, and decreased silique number. Moreover, overexpression of FviBAG6A in Fragaria vesca (2 n = 2x=14) led to a greatly reduced seed number, with nearly 80% of the seeds aborted. Analyses of paraffin sections and reactive oxygen species (ROS) content revealed that the majority of severe pollen defects were likely due to the early degradation of the tapetum and middle layer as a result of ROS accumulation and abnormal development of the uninucleate megaspore mother. Moreover, the FviBAG6-A interact with the E3 ligase SIZ1 and contribute to the SUMOylation of FviBAG6-A , which may be induced by the high level of ROS content, further promoting gametophyte abortion in strawberry transgenic lines. This study characterized the FviBAG6-A and reveals its novel function in gametophyte development.

Automated summary

This study characterized the gene FviBAG6-A and its interaction with S-RNase, revealing its role in the ubiquitin-proteasome system and gametophyte development. Overexpression of FviBAG6-A in Arabidopsis and Fragaria led to reduced fruit length, silique number, and seed number. The abnormal development of gametophytes was attributed to ROS accumulation and degradation of tapetum and middle layer, which was further influenced by the interaction of FviBAG6-A with the E3 ligase SIZ1 and ROS content.