Molecular mechanisms of toxicity and detoxification in rice (Oryza sativa L.) exposed to polystyrene nanoplastics

Nanoplastics (NPs) are an emerging threat to higher plants in terrestrial ecosystems. However, the molecular of NP-related phytotoxicity remains unclear. In the present study, rice seedlings were exposed to polystyrene (PS, 50 nm) NPs at 0, 50, 100, and 200 mg/L under hydroponic conditions to investigate the induced physiological indices and transcriptional mechanisms. We found that 50, 100, and 200 mg/L PS significantly reduced root (53.05%, 49.61%, and 57.58%, respectively) and shoot (54.63%, 61.56%, and 62.64%, respectively) biomass as compared with the control seedlings. The activities of antioxidant enzymes, including catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and ascorbate peroxidase (APX), were significantly activated in all PS treatment groups, indicating that PS inhibited plant growth and induced oxidative stress. Transcriptome analyses showed that PS modulated the expression of the genes involved in cell detoxification, active oxygen metabolism, mitogen-activated protein kinase (MAPK), and plant hormone transduction pathways. Our study provides new insights into phytotoxicity by demonstrating the potential underlying toxicity of PS NPs in higher plants.

Automated summary

In this study, rice seedlings were exposed to polystyrene nanoparticles (PS NPs) and the effects on plant physiology and gene expression were investigated. The results showed that PS NPs significantly reduced root and shoot biomass and activated antioxidant enzymes. Transcriptome analysis revealed that PS NPs modulated gene expression involved in detoxification, active oxygen metabolism, MAPK, and plant hormone transduction pathways. This study provides new insights into the phytotoxicity of PS NPs in higher plants.