CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa

Article Plant Sciences

Characterization of Squamosa-Promoter Binding Protein-Box Family Genes Reveals the Critical Role of MsSPL20 in Alfalfa Flowering Time Regulation

Lin Ma et al.

Summary: In this study, a systematic analysis of SPL genes in the alfalfa genome was conducted, revealing abundant structural variations among MsSPL genes. Through characterization of the temporal and spatial expression patterns of MsSPL genes and their response to various treatments, candidate SPL genes in alfalfa development and abiotic stress tolerant regulation were identified. Additionally, overexpression of the alfalfa-specific SPL gene (MsSPL20) led to stable delayed flowering time and increased biomass, suggesting its potential utility in molecular-assisted breeding for alfalfa.

FRONTIERS IN PLANT SCIENCE (2022)

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Article Biotechnology & Applied Microbiology

Rapid and cloning-free screening of edited alfalfa via next-generation sequencing

Margarita Stritzler et al.

Summary: This study demonstrates that next-generation sequencing can significantly reduce the time required for the identification of edited alleles in polyploid crops with complex genetics such as alfalfa. Through the analysis of fifteen edited events containing different doses of edited alleles, it was found that the use of NGS technology greatly improves the efficiency of determining the genotype of individual plants, which is attributed to the high output of sequences produced by this alternative genotyping approach.

PLANT CELL TISSUE AND ORGAN CULTURE (2022)

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Article Agronomy

Efficient CRISPR/Cas9 Genome Editing in Alfalfa Using a Public Germplasm

Emilia Bottero et al.

Summary: Tetraploid alfalfa plays a significant role as a source of vegetable proteins in meat and milk production systems in temperate regions due to its ability to acquire large amounts of nitrogen by symbiosis. Efficiency of alfalfa mutagenesis using the CRISPR/Cas9 system was demonstrated in this study, showing potential for alfalfa improvement through genome editing technologies.

FRONTIERS IN AGRONOMY (2021)

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Article Biotechnology & Applied Microbiology