Root exudates and rhizosphere microbiomes jointly determine temporal shifts in plant-soil feedbacks

Plants influence numerous soil biotic factors that can alter the performance of later growing plants-defined as plant-soil feedback (PSF). Here, we investigate whether PSF effects are linked with the temporal changes in root exudate diversity and the rhizosphere microbiome of two common grassland species (Holcus lanatus and Jacobaea vulgaris). Both plant species were grown separately establishing conspecific and heterospecific soils. In the feedback phase, we determined plant biomass, measured root exudate composition, and characterised rhizosphere microbial communities weekly (eight time points). Over time, we found a strong negative conspecific PSF on J. vulgaris in its early growth phase which changed into a neutral PSF, whereas H. lanatus exhibited a more persistent negative PSF. Root exudate diversity increased considerably over time for both plant species. Rhizosphere microbial communities were distinct in conspecific and heterospecific soils and showed strong temporal patterns. Bacterial communities converged over time. Using path models, PSF effects could be linked to the temporal dynamics of root exudate diversity, whereby shifts in rhizosphere microbial diversity contributed to temporal variation in PSF to a lesser extent. Our results highlight the importance of root exudates and rhizosphere microbial communities in driving temporal changes in the strength of PSF effects.

Automated summary

Plants influence soil biotic factors and alter the performance of later growing plants, known as plant-soil feedback (PSF). This study investigates the link between PSF effects and temporal changes in root exudate diversity and rhizosphere microbiome. The results show that root exudate diversity increases over time, and rhizosphere microbial communities differ in conspecific and heterospecific soils and exhibit strong temporal patterns. English Summary: Root exudates and rhizosphere microbial communities play an important role in driving temporal changes in the strength of plant-soil feedback effects.