期刊
PHYTOCHEMICAL ANALYSIS
卷 34, 期 4, 页码 453-460出版社
WILEY
DOI: 10.1002/pca.3226
关键词
Camptothecin; CPT; fluorescence microscopy; Nothapodytes nimmoniana
The study aimed to investigate the distribution of Camptothecin (CPT) in different parts of Nothapodytes nimmoniana and compare the efficiency of different extraction methods. The results showed that the root had the highest accumulation of CPT, while the leaves, twigs, and stembark had lower accumulation. The ultrasonic-assisted extraction with 90% methanol yielded the highest amount of CPT in the roots.
IntroductionNothapodytes nimmoniana (Icacinaceae) is a rich source of Camptothecin (CPT), an anti-cancer prodrug. Efficient extraction of CPT from various plant parts is crucial for better recovery of this pre-drug. ObjectivesTo investigate the distribution of CPT in plant parts and to compare the methods of extraction on CPT yield to evaluate how cellular localisation affects the efficiency of extraction methods. MethodsTransverse sections of plant parts were observed under a ultraviolet (UV)-fluorescence microscope for the fluorescence that the CPT molecule emits when exposed to UV radiation. Dried plant parts were extracted using 90% methanol with ultrasonic assistance, hot ethanol (61% ethanol at 60 degrees C), and chloroform-methanol (4:1, v/v). The CPT in plant parts were detected by thin-layer chromatography (TLC) and confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-performance liquid chromatography (HPLC). Quantification was carried out by HPLC. ResultsBlue fluorescence indicated a prominent accumulation of CPT in roots compared to leaf with petiole, twigs, and stembark. This accumulation was observed in upper and lower epidermis of the leaf, isolated strands of fibres in the phloem in the petiole, and groups of idioblast cells in the cortex. The ultrasonic-assisted extraction with 90% methanol showed the highest CPT yield in the root (1.91 +/- 0.02 mg/g of dry weight), followed by stembark and the least in leaves [0.02 +/- 0.01 mg/g (dry weight)] irrespective of the method of extraction. However, hot ethanol extraction gave the highest CPT yield for twig and leaf, indicating the necessity of tissue-specific extraction methods for better recovery of CPT.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据