期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 78, 期 1, 页码 105-108出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.15-0239
关键词
detection; feline; feline morbillivirus; reverse transcription loop-mediated isothermal amplification
资金
- Japan Society for the Promotion of Science
Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID50)/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID50 per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV.
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