Toxicity of Bisphenol A and its Replacements in the Mice Leydig Cells In Vitro

The aim of the study was to examine the potential impacts of bisphenol A (BPA) and its analogues BPB, BPF, and BPS on mice TM3 Leydig cells, with respect to basal cell viability parameters such as metabolic activity, cell membrane integrity, and lysosomal activity after 48-h exposure. In addition, monitoring of potential bisphenol ' s actions included evaluation of ROS production and gap junctional intercellular communication (GJIC) complemented by determination of testosterone secretion. activity started at 10 pg/ml of bisphenols after 48-h exposure. BPA and BPF and 10, 25, and 50 pg/ml of BPA and BPS. The ROS was recorded mainly at 5 and 10 pg/ml of tested compounds. In addition, significant inhibition of GJIC was a significant decline was confirmed at 10, 25 and 50 pg/ml.

Automated summary

The aim of this study was to investigate the potential impacts of bisphenol A (BPA) and its analogues BPB, BPF, and BPS on mice TM3 Leydig cells, focusing on basal cell viability parameters such as metabolic activity, cell membrane integrity, and lysosomal activity after 48-h exposure. The study also evaluated the effects of bisphenols on ROS production, gap junctional intercellular communication (GJIC), and testosterone secretion. The results showed that activity started at 10 pg/ml of bisphenols after 48-h exposure, and significant ROS production was observed at 5 and 10 pg/ml. Moreover, a significant inhibition of GJIC was confirmed at 10, 25, and 50 pg/ml.