Chlorophyll triplet states in thylakoid membranes of Acaryochloris marina. Evidence for a triplet state sitting on the photosystem I primary donor populated by intersystem crossing

Photo-induced triplet states in the thylakoid membranes isolated from the cyanobacterium Acaryocholoris marina, that harbours Chlorophyll (Chl) d as its main chromophore, have been investigated by Optically Detected Magnetic Resonance (ODMR) and time-resolved Electron Paramagnetic Resonance (TR-EPR). Thylakoids were subjected to treatments aimed at poising the redox state of the terminal electron transfer acceptors and donors of Photosystem II (PSII) and Photosystem I (PSI), respectively. Under ambient redox conditions, four Chl d triplet populations were detectable, identifiable by their characteristic zero field splitting parameters, after deconvolution of the Fluorescence Detected Magnetic Resonance (FDMR) spectra. Illumination in the presence of the redox mediator N,N,N',N'-Tetramethyl-p-phenylenediamine (TMPD) and sodium ascorbate at room temperature led to a redistribution of the triplet populations, with-T-3 (vertical bar D vertical bar= 0.0245-cm(-1), vertical bar E vertical bar= 0.0042-cm(-1)) becoming dominant and increasing in intensity with respect to untreated samples. A second triplet population -(T-4, vertical bar D vertical bar = 0.0248-cm(-1), vertical bar E vertical bar= 0.0040-cm(-1)) having an intensity ratio of about 1:4 with respect to -T3 was also detectable after illumination in the presence of TMPD and ascorbate. The microwave-induced Triplet-minus-Singlet spectrum acquired at the maximum of the vertical bar D vertical bar-vertical bar E vertical bar transition (610 MHz) displays a broad minimum at 740 nm, accompanied by a set of complex spectral features that overall resemble, despite showing further fine spectral structure, the previously reported Triplet-minus-Singlet spectrum attributed to the recombination triplet of PSI reaction centre, 3P 740 [Schenderlein M, Cetin M, Barber J, et al. Spectroscopic studies of the chlorophyll d containing photosystem I from the cyanobacterium Acaryochloris marina. Biochim Biophys Acta 1777:1400-1408]. However, TR-EPR experiments indicate that this triplet displays an eaeaea electron spin polarisation pattern which is characteristic of triplet sublevels populated by intersystem crossing rather than recombination, for which an aeeaae polarisation pattern is expected instead. It is proposed that the observed triplet, which leads to the bleaching of the P-740 singlet state, sits on the PSI reaction centre.

Automated summary

Photo-induced triplet states in the thylakoid membranes of Acaryocholoris marina, a cyanobacterium with Chl d as its main chromophore, were investigated using ODMR and TR-EPR. Four Chl d triplet populations were detected under ambient redox conditions, and-T3 became dominant after illumination with TMPD and ascorbate. Another triplet population -T4 was also observed with an intensity ratio of 1:4 compared to -T3. TR-EPR experiments indicated that these triplets were formed by intersystem crossing and bleached the P-740 singlet state in the PSI reaction center.