期刊
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
卷 42, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.pdpdt.2023.103520
关键词
Cationic phthalocyanines; Biofilms; Photodynamic antimicrobial therapy; Raman spectroscopy; Cyclic voltammetry; Scanning electron microscopy
类别
In this study, we synthesized three phthalocyanine complexes and investigated their effects on photodynamic antimicrobial chemotherapy (PACT) against E.coli and S.aureus in both planktonic and biofilm forms. Complex 4, with 8 cationic charges, showed the highest activity against the biofilms. Surface stability of E.coli and S.aureus biofilms in the presence of complex 4 was analyzed using cyclic voltammetry, and conformational and biochemical changes within the biofilm were studied using scanning electron microscopy (SEM) and Raman spectra.
In this work, we have described the synthesis of phthalocyanine complexes Zn(II) tetrakis 4-(5-formylpyridin-2-yl)oxy) phthalocyanine (2), Zn(II) tetrakis-1-butyl-4-(2-(6-(tetra-phenoxy)pyridin-3-yl) vinyl)pyridin-1-ium phthalocyanine (3) and Zn(II) tetrakis 1-butyl-5-(2-(1-butylpyridin-1-ium-4-yl)vinyl)-2-(tetra-phenoxy)pyridin-1-ium phthalocyanine (4). The effect of a varying number of charges when the Pc complexes are alone or grafted in gallic acid (GA) tagged silica nanoparticles on photodynamic antimicrobial chemotherapy (PACT) is inves-tigated toward Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) in both planktonic and biofilm forms. Complex 4, bearing a total of 8 cationic charges, displayed the highest activity with log CFU (colony forming units) values of 8.60 and 6.42 against E.coli and S.aureus biofilms, respectively. The surface stability of E.coli and S.aureus biofilms in the presence of 4 and its conjugate was analyzed using cyclic voltammetry. Scanning electron microscopy (SEM) and Raman spectra are also used to study the conformational and biochemical changes within biofilm upon subjecting them to PACT.
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