期刊
PHARMACEUTICAL RESEARCH
卷 40, 期 4, 页码 937-949出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11095-023-03482-4
关键词
discovery; drug classification; intrinsic clearance; in-vitro; permeability
In this study, the applicability of the Extended Clearance Concept Classification System (EC3S) in drug discovery was investigated. A relationship between hepatocyte uptake clearance, MDCK permeability, and intrinsic clearance from human liver microsomes was established. The simplified EC3S was able to predict systemic clearance for certain compounds.
PurposeThe Extended Clearance Concept Classification System was established as a development-stage tool to provide a framework for identifying fundamental mechanism(s) governing drug disposition in humans. In the present study, the applicability of the EC3S in drug discovery has been investigated. In its current format, the EC3S relies on low-throughput hepatocyte uptake data, which are not frequently generated in a discovery setting.MethodsA relationship between hepatocyte uptake clearance and MDCK permeability was first established along with intrinsic clearance from human liver microsomes. The performance of this approach was examined by categorizing 64 drugs into EC3S classes and comparing the predicted major elimination pathway(s) to that observed in humans. As an extension of the work, the ability of the simplified EC3S to predict human systemic clearance based on intrinsic clearance generated using in-vitro metabolic systems was evaluated.ResultsThe assessment enabled the use of MDCK permeability and unscaled unbound intrinsic clearance to generate cut-off criteria to categorize compounds into four EC3S classes: Class 12ab, 2cd, 34ab, and 34cd, with major elimination mechanism(s) assigned to each class. The predictivity analysis suggested that systemic clearance could generally be predicted within threefold for EC3S class 12ab and 34ab compounds. For classes 2cd and 34cd, systemic clearance was poorly predicted using in-vitro systems explored in this study.ConclusionCollectively, our simplified classification approach is expected to facilitate the identification of mechanism(s) involved in drug elimination, faster resolution of in-vitro to in-vivo disconnects, and better design of mechanistic pharmacokinetic studies in drug discovery.
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