Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta

BACKGROUNDIndoxacarb, representing an efficient insecticide, is normally made into a bait to spread the poison among red fire ants so that it can be widely applied in the prevention and control of Solenopsis invicta. However, the potential toxicity mechanism of S. invicta in response to indoxacarb remains to be explored. In this study, we integrated mass spectrometry imaging (MSI) and untargeted metabolomics methods to reveal disturbed metabolic expression levels and spatial distribution within the whole-body tissue of S. invicta treated with indoxacarb. RESULTSMetabolomics results showed a significantly altered level of metabolites after indoxacarb treatment, such as carbohydrates, amino acids and pyrimidine and derivatives. Additionally, the spatial distribution and regulation of several crucial metabolites resulting from the metabolic pathway and lipids can be visualized using label-free MSI methods. Specifically, xylitol, aspartate, and uracil were distributed throughout the whole body of S. invicta, while sucrose-6 '-phosphate and glycerol were mainly distributed in the abdomen of S. invicta, and thymine was distributed in the head and chest of S. invicta. Taken together, the integrated MSI and metabolomics results indicated that the toxicity mechanism of indoxacarb in S. invicta is closely associated with the disturbance in several key metabolic pathways, such as pyrimidine metabolism, aspartate metabolism, pentose and glucuronate interconversions, and inhibited energy synthesis. CONCLUSIONCollectively, these findings provide a new perspective for the understanding of toxicity assessment between targeted organisms S. invicta and pesticides. (c) 2023 Society of Chemical Industry.

Automated summary

This study investigated the metabolic changes in red fire ants treated with indoxacarb using mass spectrometry imaging and untargeted metabolomics methods. The results showed significant alterations in metabolite levels, including carbohydrates, amino acids, and pyrimidine and derivatives. The label-free MSI method visualized the spatial distribution and regulation of key metabolites, revealing the association between indoxacarb toxicity and disturbance in metabolic pathways.