Laboratory diagnosis of HIV: a contemporary overview in the Australian context

Over the past decade there have been technical advances in human immunodeficiency virus (HIV) assays and up -dates to testing regulations that have substantially changed the landscape of laboratory testing for HIV. In addition, there have been significant changes in the epidemiology of HIV in Australia in the context of highly effective contemporary biomedical treatment and preven-tion strategies. Here, we provide an update on contem-porary issues for the laboratory detection and confirmation of HIV in Australia. These include (1) the impact of early treatment and biological prevention strategies on the serological and virological detection of HIV; (2) the upda-ted national HIV laboratory case definition and its inter-action with testing regulations, public health and clinical guidelines; and (3) novel strategies for the laboratory detection of HIV, including the incorporation of HIV nucleic acid amplification tests (NAATs) into testing algorithms. These developments present an opportunity to develop a nationally consistent contemporary HIV testing algorithm that would result in optimisation and standardisation of HIV testing in Australia.

Automated summary

In the past decade, advancements in HIV detection technology and testing regulations have greatly impacted laboratory testing for HIV in Australia. This article provides an update on the current issues concerning the laboratory detection and confirmation of HIV, including the impact of early treatment and prevention strategies, updated national HIV laboratory case definition, and novel strategies for HIV detection. These developments create an opportunity to develop a nationally consistent contemporary HIV testing algorithm for optimization and standardization of HIV testing in Australia.