High-accuracy differential autofocus system with an electrically tunable lens

Article Biotechnology & Applied Microbiology

Three-dimensional structured illumination microscopy with enhanced axial resolution

Xuesong Li et al.

Summary: Enhanced axial resolution for 3D SIM is achieved with deep learning or four-beam interference. These methods allow for higher spatial frequency content and near-isotropic imaging with resolutions of around 120 nm in both lateral and axial directions. The deep learning method can also be combined with denoising for volumetric imaging. Various cellular samples were imaged to demonstrate the potential of these advancements.

NATURE BIOTECHNOLOGY (2023)

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Article Chemistry, Multidisciplinary

Nanoscale Patterning of In Vitro Neuronal Circuits

Jose C. Mateus et al.

Summary: This research utilizes electron beam lithography and photolithography to create microstructures that control neuronal connectivity, and demonstrates the formation of functional synapses in vitro.

ACS NANO (2022)

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Article Physics, Applied

Theoretical design of ultracompact three-dimensional isotropic two-photon 4Pi STED microscopy based on multifunctional metalenses focusing system

Yang Li et al.

Summary: This paper proposes an ultracompact two-photon 4Pi STED microscopy using metalenses to overcome the limitations of studying synapses in deep brain tissue, and achieves three-dimensional isotropic super-resolution imaging.

JOURNAL OF PHYSICS D-APPLIED PHYSICS (2022)

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Review Chemistry, Multidisciplinary

Lanthanide-Doped Near-Infrared Nanoparticles for Biophotonics

Hui Li et al.

Summary: Light in the near-infrared (NIR) spectral region is increasingly used in bioapplications due to its deeper penetration in biological tissues. Lanthanide-doped luminescent nanoparticles with excitation and/or emission in the NIR range are gaining attention for noninvasive biological applications, offering unique optical properties such as large Stokes shift, sharp luminescence emissions, long lifetimes, and photostability. Advanced strategies for enhancing the luminescence brightness of these nanoparticles have been introduced, along with a review of their superior biophotonic applications in high-resolution imaging, single-nanoparticle-level detection, and tissue-penetrating diagnostics and therapeutics.

ADVANCED MATERIALS (2021)

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Article Biochemistry & Molecular Biology

An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice

Jin-Fan Zhang et al.

Summary: A new ultrasensitive protein kinase A activity reporter has been developed for tracking kinase activity dynamics across various experimental contexts. Results from in vivo visual cortex imaging in awake mice show highly dynamic neuronal PKA activity rapidly recruited by forced locomotion.

NATURE CHEMICAL BIOLOGY (2021)

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Article Physiology