期刊
ONCOLOGY REPORTS
卷 49, 期 5, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2023.8526
关键词
colorectal cancer; NONHSAG028908; 3; microRNA-34a-5p; aldolase; fructose-bisphosphate A
类别
The aim of this study was to investigate the regulatory mechanism of lncRNA NONHSAG028908.3 on CRC. The results showed that silencing of NONHSAG028908.3 inhibited proliferation, migration, and invasion of CRC cells. Furthermore, NONHSAG028908.3 regulated ALDOA expression by sponging miR-34a-5p, promoting malignant activities in CRC.
Colorectal cancer (CRC) is an aggressive tumor, whose development is considered to be modulated by certain long non-coding RNAs (lncRNAs). Therefore, the aim of the present study was to investigate the regulatory mechanism of lncRNA NONHSAG028908.3 on CRC. Data from The Cancer Genome Atlas (TCGA) database revealed that NONHSAG028908.3 was increased in CRC tissues compared with normal tissues (P<0.001). The results of reverse transcription-quantitative PCR indicated that NONHSAG028908.3 was upregulated in four types of CRC cells compared with that in NCM460, a normal colorectal cell line. MTT, BrdU, and flow cytometric assays were applied to evaluate CRC cell growth. The migratory and invasive abilities of CRC cells were detected using wound healing and Transwell assays. Silencing of NONHSAG028908.3 inhibited proliferation, migration, and invasion of CRC cells. A dual-luciferase reporter assay demonstrated that NONHSAG028908.3 served as a sponge to combine with microRNA (miR)-34a-5p. MiR-34a-5p suppressed the aggressiveness of CRC cells. The effects induced by NONHSAG028908.3 knockdown were partly reversed by inhibition of miR-34a-5p. Furthermore, miR-34a-5p, a target of NONHSAG028908.3, modulated aldolase, fructose-bisphosphate A (ALDOA) expression in a negative feedback manner. Suppression of NONHSAG028908.3 notably decreased ALDOA expression, which was rescued via silencing of miR-34a-5p. Moreover, suppression of ALDOA revealed the inhibitory action on CRC cell growth and migration. In summary, the data of the present study indicate that NONHSAG028908.3 may positively regulate ALDOA via sponging miR-34a-5p, thereby promoting malignant activities in CRC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据