期刊
ONCOLOGY REPORTS
卷 50, 期 2, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2023.8589
关键词
N-Myc downstream regulatory gene 3; beta-catenin; chronic myelogenous leukemia; imatinib; microRNA-204-5p
类别
This study found that NDRG3 is highly expressed in patients with chronic myelogenous leukemia (CML), promoting cell proliferation and enhancing imatinib resistance. MiR-204-5p inhibits the expression of NDRG3, while beta-catenin plays a role in cell proliferation and drug resistance. These findings provide theoretical support for overcoming drug resistance in CML.
Imatinib resistance in chronic myelogenous leukemia (CML) is a clinical problem. The present study examined the role of N-Myc downstream regulatory gene 3 (NDRG3) in imatinib resistance in CML. Quantitative PCR demonstrated that NDRG3 was highly expressed in patients with CML. Cell Counting Kit (CCK)-8 experiments proved that NDRG3 promoted the proliferation of K562 CML cells and enhanced imatinib resistance. Dual-luciferase assay showed that microRNA (miR)-204-5p inhibited expression of NDRG3 and immunofluorescence experiments showed that NDRG3 promoted accumulation of beta-catenin in the nucleus, thereby increasing the expression of downstream drug resistance- and cell cycle-associated factors (c-Myc and MDR1). At the same time, cell proliferation experiments showed that beta-catenin played a role in cell proliferation and drug resistance. Co-transfection with small interfering (si)-beta-catenin partially reversed the effect of NDRG3. This finding indicated that NDRG3 plays an important role in imatinib resistance and miR-204-5p and beta-catenin are involved in the biological behavior of NDRG3. The present results provide theoretical support for overcoming drug resistance in CML.
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