Spatiotemporal regulation of cholangiocarcinoma growth and dissemination by peritumoral myofibroblasts in a Vcam1-dependent manner

Intrahepatic cholangiocarcinoma (iCCA) is characterized by its highly desmoplastic stroma. Myofibroblasts (MFs) are present both within the tumor mass (intratumoral MFs, iMFs) and at the tumor border (peritumoral MFs, pMFs). Using a spheroid-based coculture system, we show that the initial iCCA-pMF contact is growth suppressive to the tumor cells. However, prolonged iCCA-pMF interaction elicits significant tumor cell invasion and dissemination. We find that vascular cell adhesion molecule-1 (Vcam1) level is elevated in tumor cells in contact with pMFs but low in disseminated tumor cells both in vitro and in vivo. A gene regulatory network analysis of mouse and patient iCCA tumors and Vcam1 knockout (Vcam1(KO)) demonstrate a heavy involvement of Vcam1 in epithelial-to-mesenchymal transition. While Vcam1(KO) has only a limited impact on tumor cell growth in their monoculture, Vcam1(KO) spheroids exhibit instant dissemination and a severe growth defect when cocultured with pMFs. When transplanted into the liver, Vcam1(KO) iCCA cells show a similar increase in dissemination but a significant defect in establishing primary and metastatic tumors. Incomplete blocking of Vcam1 in vivo reduces the size but increase the number of metastatic lesions. Overall, our study shows a spatiotemporal regulation of iCCA growth and dissemination by pMFs in a Vcam1-dependent manner.

Automated summary

Intrahepatic cholangiocarcinoma (iCCA) is characterized by highly desmoplastic stroma. Contact between tumor cells and peritumoral myofibroblasts (pMFs) initially suppresses tumor cell growth but promotes invasion and dissemination in the long term. Vascular cell adhesion molecule-1 (Vcam1) plays a significant role in this process by regulating epithelial-to-mesenchymal transition. Overall, this study reveals the spatiotemporal regulation of iCCA growth and dissemination by pMFs in a Vcam1-dependent manner.