期刊
NUCLEIC ACIDS RESEARCH
卷 51, 期 14, 页码 7342-7356出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkad515
关键词
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Cockayne syndrome protein B (CSB) collaborates with PARP1 in repairing oxidized DNA. CSB is recruited to damaged DNA by PARP1 and PARP2, and regulates single-strand break repair (SSBR). CSB-mediated SSBR primarily occurs at actively transcribed DNA regions, suggesting different mechanisms for SSBR based on transcription status.
Efficient repair of oxidized DNA is critical for genome-integrity maintenance. Cockayne syndrome protein B (CSB) is an ATP-dependent chromatin remodeler that collaborates with Poly(ADP-ribose) polymerase I (PARP1) in the repair of oxidative DNA lesions. How these proteins integrate during DNA repair remains largely unknown. Here, using chromatin co-fractionation studies, we demonstrate that PARP1 and PARP2 promote recruitment of CSB to oxidatively-damaged DNA. CSB, in turn, contributes to the recruitment of XRCC1, and histone PARylation factor 1 (HPF1), and promotes histone PARylation. Using alkaline comet assays to monitor DNA repair, we found that CSB regulates single-strand break repair (SSBR) mediated by PARP1 and PARP2. Strikingly, CSB's function in SSBR is largely bypassed when transcription is inhibited, suggesting CSB-mediated SSBR occurs primarily at actively transcribed DNA regions. While PARP1 repairs SSBs at sites regardless of the transcription status, we found that PARP2 predominantly functions in actively transcribed DNA regions. Therefore, our study raises the hypothesis that SSBR is executed by different mechanisms based on the transcription status.
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