期刊
NUCLEIC ACIDS RESEARCH
卷 51, 期 8, 页码 4000-4011出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkad144
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Two types of glycyl-tRNA synthetase (GlyRS) have been identified, alpha(2) and alpha(2)beta(2) GlyRSs. While the mechanisms for alpha(2) GlyRSs are well understood, little is known about the mechanisms for alpha(2)beta(2)/(alpha beta)(2) GlyRSs. This study presents the structures of the (alpha beta)(2) GlyRS from Oryza sativa chloroplast, revealing a complex mechanism of tRNA selection and access to the active site.
Two types of glycyl-tRNA synthetase (GlyRS) are known, the alpha(2) and the alpha(2)beta(2) GlyRSs. Both types of synthetase employ a class II catalytic domain to aminoacylate tRNA(Gly). In plastids and some bacteria, the alpha and beta subunits are fused and are designated as (alpha beta)(2) GlyRSs. While the tRNA recognition and aminoacylation mechanisms are well understood for alpha(2) GlyRSs, little is known about the mechanisms for alpha(2)beta(2)/(alpha beta)(2) GlyRSs. Here we describe structures of the (alpha beta)(2) GlyRS from Oryza sativa chloroplast by itself and in complex with cognate tRNA(Gly). The set of structures reveals that the U-shaped beta half of the synthetase selects the tRNA in a two-step manner. In the first step, the synthetase engages the elbow and the anticodon base C35 of the tRNA. In the second step, the tRNA has rotated similar to 9 degrees toward the catalytic centre. The synthetase probes the tRNA for the presence of anticodon base C36 and discriminator base C73. This intricate mechanism enables the tRNA to access the active site of the synthetase from a direction opposite to that of most other class II synthetases.
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