Core defense hotspots within Pseudomonas aeruginosa are a consistent and rich source of anti-phage defense systems

Bacteria use a diverse arsenal of anti-phage immune systems, including CRISPR-Cas and restriction enzymes. Recent advances in anti-phage system discovery and annotation tools have unearthed many unique systems, often encoded in horizontally transferred defense islands, which can be horizontally transferred. Here, we developed Hidden Markov Models (HMMs) for defense systems and queried microbial genomes on the NCBI database. Out of the 30 species with >200 completely sequenced genomes, our analysis found Pseudomonas aeruginosa exhibits the greatest diversity of anti-phage systems, as measured by Shannon entropy. Using network analysis to identify the common neighbors of anti-phage systems, we identified two core defense hotspot loci (cDHS1 and cDHS2). cDHS1 is up to 224 kb (median: 26 kb) with varied arrangements of more than 30 distinct immune systems across isolates, while cDHS2 has 24 distinct systems (median: 6 kb). Both cDHS regions are occupied in a majority of P. aeruginosa isolates. Most cDHS genes are of unknown function potentially representing new anti-phage systems, which we validated by identifying a novel anti-phage system (Shango) commonly encoded in cDHS1. Identifying core genes flanking immune islands could simplify immune system discovery and may represent popular landing spots for diverse MGEs carrying anti-phage systems.

Automated summary

Bacteria have a wide range of anti-phage immune systems, including CRISPR-Cas and restriction enzymes. Hidden Markov Models were developed to analyze defense systems in microbial genomes and it was found that Pseudomonas aeruginosa has the highest diversity of anti-phage systems. Two core defense hotspots, cDHS1 and cDHS2, were identified and these regions are commonly found in P. aeruginosa isolates. The discovery of new anti-phage systems in these regions could simplify immune system research.