期刊
NUCLEIC ACIDS RESEARCH
卷 51, 期 W1, 页码 W484-W492出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkad326
关键词
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Proksee is a powerful and user-friendly system for assembling, annotating, analyzing, and visualizing bacterial genomes. It accepts various file formats and provides unique assembly metrics, a high-performance genome browser, embedded analysis tools, and the option to export data for sharing and reproducibility. The system is designed to scale and ensure a robust and responsive web server.
Proksee (https://proksee.ca) provides users with a powerful, easy-to-use, and feature-rich system for assembling, annotating, analysing, and visualizing bacterial genomes. Proksee accepts Illumina sequence reads as compressed FASTQ files or pre assembled contigs in raw, FASTA, or GenBank format. Alternatively, users can supply a GenBank accession or a previously generated Proksee map in JSON format. Proksee then performs assembly (for raw sequence data), generates a graphical map, and provides an interface for customizing the map and launching further analysis jobs. Notable features of Proksee include unique and informative assembly metrics provided via a custom reference database of assemblies; a deeply integrated high-performance genome browser for viewing and comparing analysis results at individual base resolution (developed specifically for Proksee); an ever-growing list of embedded analysis tools whose results can be seamlessly added to the map or searched and explored in other formats; and the option to export graphical maps, analysis results, and log files for data sharing and research reproducibility. All these features are provided via a carefully designed multi-server cloud based system that can easily scale to meet user demand and that ensures the web server is robust and responsive.
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