Near-physiological in vitro assembly of 50S ribosomes involves parallel pathways

Understanding the assembly principles of biological macromolecular complexes remains a significant challenge, due to the complexity of the systems and the difficulties in developing experimental approaches. As a ribonucleoprotein complex, the ribosome serves as a model system for the profiling of macromolecular complex assembly. In this work, we report an ensemble of large ribosomal subunit intermediate structures that accumulate during synthesis in a near-physiological and co-transcriptional in vitro reconstitution system. Thirteen pre-50S intermediate maps covering the entire assembly process were resolved using cryo-EM single-particle analysis and heterogeneous subclassification. Segmentation of the set of density maps reveals that the 50S ribosome intermediates assemble based on fourteen cooperative assembly blocks, including the smallest assembly core reported to date, which is composed of a 600-nucleotide-long folded rRNA and three ribosomal proteins. The cooperative blocks assemble onto the assembly core following defined dependencies, revealing the parallel pathways at both early and late assembly stages of the 50S subunit.

Automated summary

We investigated the assembly principles of 50S ribosomal subunits using cryo-EM and found that they assemble based on cooperative assembly blocks and defined dependencies. We resolved 13 intermediate structures that accumulate during synthesis, revealing the smallest known assembly core. Our findings shed light on the parallel pathways involved in the early and late assembly stages of the 50S subunit.