期刊
NUCLEIC ACIDS RESEARCH
卷 51, 期 11, 页码 5341-5350出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkad434
关键词
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Photochemical dimerization of adjacent pyrimidines is crucial for the creation of mutagenic hotspots, and the distribution of resulting lesions (CPDs) is highly variable in cells due to DNA conformation. Previous studies have mainly focused on mechanisms influencing CPD formation without considering CPD reversion. However, this report demonstrates that reversion is competitive under standard irradiation conditions and suggests that both CPD formation and reversion control CPD populations before reaching photo-steady-state conditions.
Photochemical dimerization of adjacent pyrimidines is fundamental to the creation of mutagenic hotspots caused by ultraviolet light. Distribution of the resulting lesions (cyclobutane pyrimidine dimers, CPDs) is already known to be highly variable in cells, and in vitro models have implicated DNA conformation as a major basis for this observation. Past efforts have primarily focused on mechanisms that influence CPD formation and have rarely considered contributions of CPD reversion. However, reversion is competitive under the standard conditions of 254 nm irradiation as illustrated in this report based on the dynamic response of CPDs to changes in DNA conformation. A periodic profile of CPDs was recreated in DNA held in a bent conformation by lambda repressor. After linearization of this DNA, the CPD profile relaxed to its characteristic uniform distribution over a similar time of irradiation to that required to generate the initial profile. Similarly, when a T tract was released from a bent conformation, its CPD profile converted under further irradiation to that consistent with a linear T tract. This interconversion of CPDs indicates that both its formation and reversion exert control on CPD populations long before photo-steady-state conditions are achieved and suggests that the dominant sites of CPDs will evolve as DNA conformation changes in response to natural cellular processes.
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