期刊
NEUROCHEMICAL RESEARCH
卷 48, 期 8, 页码 2490-2501出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-023-03921-z
关键词
Nucleus accumbens shell; Ventral subiculum; RNASeq; Retro-TRAP; Ribosomal bound; Retrograde tracing
The nucleus accumbens shell is an important part of the reward circuit, responsible for encoding reward-associated environments. This study identified the ventral subiculum as the brain region with the highest glutamatergic input to the shell. By analyzing the molecular characteristics of different glutamatergic projections from the ventral subiculum to the nucleus accumbens shell, the study revealed differential gene enrichment and highlighted potential differences in neuronal projections.
The nucleus accumbens shell is a critical node in reward circuitry, encoding environments associated with reward. Long-range inputs from the ventral hippocampus (ventral subiculum) to the nucleus accumbens shell have been identified, yet their precise molecular phenotype remains to be determined. Here we used retrograde tracing to identify the ventral subiculum as the brain region with the densest glutamatergic (VGluT1-Slc17a7) input to the shell. We then used circuit-directed translating ribosome affinity purification to examine the molecular characteristics of distinct glutamatergic (VGluT1, VGluT2-Slc17a6) ventral subiculum to nucleus accumbens shell projections. We immunoprecipitated translating ribosomes from this population of projection neurons and analysed molecular connectomic information using RNA sequencing. We found differential gene enrichment across both glutamatergic projection neuron subtypes. In VGluT1 projections, we found enrichment of Pfkl, a gene involved in glucose metabolism. In VGluT2 projections, we found a depletion of Sparcl1 and Dlg1, genes known to play a role in depression- and addiction-related behaviours. These findings highlight potential glutamatergic neuronal-projection-specific differences in ventral subiculum to nucleus accumbens shell projections. Together these data advance our understanding of the phenotype of a defined brain circuit.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据