Comparison of extruded cell nanovesicles and exosomes in their molecular cargos and regenerative potentials

Extracellular vesicles (EVs) generated from mesenchymal stem cells (MSCs) play an essential role in modulating cell cell communication and tissue regeneration. The clinical translation of EVs is constrained by the poor yield of EVs. Extrusion has recently become an effective technique for producing a large scale of nanovesicles (NVs), In this study, we systematically compared MSC NVs (from extrusion) and EVs (from natural secretion). Proteomics and RNA sequencing data revealed that NVs resemble MSCs more closely than EVs. Additionally, microRNAs in NVs are related to cardiac repair, fibrosis repression, and angiogenesis. Lastly, intravenous delivery of MSC NVs improved heart repair and cardiac function in a mouse model of myocardial infarction.

Automated summary

Extracellular vesicles (EVs) generated from mesenchymal stem cells (MSCs) play a crucial role in cell-cell communication and tissue regeneration. However, their clinical potential is limited due to low yield. This study compared MSC nanovesicles (NVs) obtained through extrusion with EVs from natural secretion. Proteomics and RNA sequencing data showed that NVs closely resemble MSCs and contain microRNAs associated with cardiac repair, fibrosis repression, and angiogenesis. Intravenous delivery of MSC NVs improved heart repair and cardiac function in a mouse model of myocardial infarction.