期刊
NANO RESEARCH
卷 16, 期 5, 页码 7459-7469出版社
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-022-5354-4
关键词
Alzheimer's disease; p-tau(396,404); plasma detection; surface-enhanced Raman scattering; lateral flow assay
Phosphorylation of tau at Ser(396,404) is an early event in Alzheimer's disease (AD), and plasma p-tau(396,404) level could be a potential biomarker for AD. Researchers developed a dual-output lateral flow assay (LFA) that combines colorimetric and surface-enhanced Raman scattering (SERS) for the rapid, sensitive, and robust detection of plasma p-tau(396,404) levels. The LFA showed a detection limit of 60 pg/mL by the naked eye or 3.8 pg/mL by SERS, with no cross-reactivity with other tau species. This LFA could differentiate AD patients from healthy controls, suggesting its potential for point-of-care AD diagnosis.
Phosphorylation of tau at Ser (396, 404) (p-tau(396,404)) is one of the earliest phosphorylation events, and plasma p-tau(396,404) level appears to be a potentially promising biomarker of Alzheimer's disease (AD). The low abundance and easy degradation of p-tau in the plasma make the lateral flow assay (LFA) a suitable choice for point-of-care detection of plasma p-tau(396,404) levels. Herein, based on our screening of a pair of p-tau(396,404)-specific antibodies, we developed a colorimetric and surface-enhanced Raman scattering (SERS) dual-readout LFA for the rapid, highly sensitive, and robust detection of plasma p-tau(396,404) levels. This LFA realized a detection limit of 60 pg/mL by the naked eye or 3.8 pg/mL by SERS without cross-reacting with other tau species. More importantly, LFA rapidly and accurately differentiated AD patients from healthy controls, suggesting that it has the potential for clinical point-of-care application in AD diagnosis. This dual-readout LFA has the advantages of simple operation, rapid, and ultra-sensitive detection, providing a new way for early AD diagnosis and intervention, especially in primary and community AD screening.
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