Comparing the Colloidal Stabilities of Commercial and Biogenic Iron Oxide Nanoparticles That Have Potential In Vitro/In Vivo Applications

For the potential in vitro/in vivo applications of magnetic iron oxide nanoparticles, their stability in different physiological fluids has to be ensured. This important prerequisite includes the preservation of the particles' stability during the envisaged application and, consequently, their invariance with respect to the transfer from storage conditions to cell culture media or even bodily fluids. Here, we investigate the colloidal stabilities of commercial nanoparticles with different coatings as a model system for biogenic iron oxide nanoparticles (magnetosomes) isolated from magnetotactic bacteria. We demonstrate that the stability can be evaluated and quantified by determining the intensity-weighted average of the particle sizes (Z-value) obtained from dynamic light scattering experiments as a simple quality criterion, which can also be used as an indicator for protein corona formation.

Automated summary

To ensure the potential applications of magnetic iron oxide nanoparticles in vitro/in vivo, their stability in different physiological fluids needs to be guaranteed. This study investigates the colloidal stabilities of commercial nanoparticles with different coatings and demonstrates that stability can be evaluated and quantified by determining the intensity-weighted average of particle sizes (Z-value). This simple quality criterion can also serve as an indicator for protein corona formation.