期刊
MOLECULES
卷 28, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/molecules28052341
关键词
arsenic(III); fluorescent aptasensor; triple-helix molecular switch; rapid detection
A novel aptamer-based fluorescent-sensing platform using a triple-helix molecular switch (THMS) was developed for detecting arsenic(III) ion. The detection process was rapid, simple, and sensitive, with a limit of detection of 69.95 nM. The THMS-based aptasensor also showed excellent stability and selectivity, and was successfully used for arsenic detection in a real water sample from Huangpu River.
A novel aptamer-based fluorescent-sensing platform with a triple-helix molecular switch (THMS) was proposed as a switch for detecting the arsenic(III) ion. The triple helix structure was prepared by binding a signal transduction probe and arsenic aptamer. Additionally, the signal transduction probe labeled with fluorophore (FAM) and quencher (BHQ1) was employed as a signal indicator. The proposed aptasensor is rapid, simple and sensitive, with a limit of detection of 69.95 nM. The decrease in peak fluorescence intensity shows a linear dependence, with the concentration of As(III) in the range of 0.1 mu M to 2.5 mu M. The whole detection process takes 30 min. Moreover, the THMS-based aptasensor was also successfully used to detect As(III) in a real sample of Huangpu River water with good recoveries. The aptamer-based THMS also presents distinct advantages in stability and selectivity. The proposed strategy developed herein can be extensively applied in the field of food inspection.
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