Thermodynamic and Kinetic Stabilities of Al(III) Complexes with N2O3 Pentadentate Ligands

Al(III) complexes have been recently investigated for their potential use in imaging with positron emission tomography (PET) by formation of ternary complexes with the radioisotope fluorine-18 (F-18). Although the derivatives of 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) are the most applied chelators for [(AlF)-F-18](2+) labelling and (pre)clinical PET imaging, non-macrocyclic, semi-rigid pentadentate chelators having two N- and three O-donor atoms such as RESCA1 and AMPDA-HB have been proposed with the aim to allow room temperature labelling of temperature-sensitive biomolecules. The paucity of stability data on Al(III) complexes used for PET imaging instigated a complete thermodynamic and kinetic solution study on Al(III) complexes with aminomethylpiperidine (AMP) derivatives AMPTA and AMPDA-HB and the comparison with a RESCA1-like chelator CD3A-Bn (trans-1,2-diaminocyclohexane-N-benzyl-N,N ',N '-triacetic acid). The stability constant of [Al(AMPDA-HB)] is about four orders of magnitude higher than that of [Al(AMPTA)] and [Al(CD3A-Bn)], highlighting the greater affinity of phenolates with respect to acetate O-donors. On the other hand, the kinetic inertness of the complexes, determined by following the Cu2+-mediated transmetallation reactions in the 7.5-10.5 pH range, resulted in a spontaneous and hydroxide-assisted dissociation slightly faster for [Al(AMPTA)] than for the other two complexes (t(1/2) = 4.5 h for [Al(AMPTA)], 12.4 h for [Al(AMPDA-HB)], and 24.1 h for [Al(CD3A-Bn)] at pH 7.4 and 25 degrees C). Finally, the [AlF](2+) ternary complexes were prepared and their stability in reconstituted human serum was determined by F-19 NMR experiments.

Automated summary

Al(III) complexes have been studied for their potential use in PET imaging by forming ternary complexes with F-18. While NOTA derivatives are commonly used for labelling and PET imaging, non-macrocyclic pentadentate chelators like RESCA1 and AMPDA-HB provide room temperature labelling for temperature-sensitive biomolecules. Thermodynamic and kinetic studies of Al(III) complexes with AMP derivatives and a RESCA1-like chelator showed higher stability of [Al(AMPDA-HB)] and faster dissociation for [Al(AMPTA)] in solution. The stability of [AlF](2+) ternary complexes in human serum was also determined.