The Development of Tungsten Biochemistry-A Personal Recollection

The development of tungsten biochemistry is sketched from the viewpoint of personal participation. Following its identification as a bio-element, a catalogue of genes, enzymes, and reactions was built up. EPR spectroscopic monitoring of redox states was, and remains, a prominent tool in attempts to understand tungstopterin-based catalysis. A paucity of pre-steady-state data remains a hindrance to overcome to this day. Tungstate transport systems have been characterized and found to be very specific for W over Mo. Additional selectivity is presented by the biosynthetic machinery for tungstopterin enzymes. Metallomics analysis of hyperthermophilic archaeon Pyrococcus furiosus indicates a comprehensive inventory of tungsten proteins.

Automated summary

The development of tungsten biochemistry is discussed, with a focus on personal involvement. A comprehensive catalog of genes, enzymes, and reactions related to tungsten has been compiled. EPR spectroscopy has played a significant role in understanding tungstopterin-based catalysis. However, the lack of pre-steady-state data remains a challenge. Tungstate transport systems and biosynthetic machinery for tungstopterin enzymes have been studied, revealing their specificity for tungsten and additional selectivity. Metallomics analysis of Pyrococcus furiosus has provided a comprehensive inventory of tungsten proteins.