Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

BackgroundEscherichia coli heat labile toxin B subunit (LTB) is one of the most popular oral vaccine adjuvants and intestine adsorption enhancers. It is often expressed as a fusion partner with target antigens to enhance their immunogenicity as well as gut absorbability. However, high expression levels of a fusion protein are critical to the outcome of immunization experiments and the success of subsequent vaccine development efforts. In order to improve the expression and functional assembly of LTB-fusion proteins using Saccharomyces cerevisiae, we compared their expression under culture conditions at a sub-physiological temperature 20 degrees C with their expression under a standard 30 degrees C.ResultsThe assembled expression of LTB-EDIII2 (LTB fused to the envelope domain III (EDIII) of Dengue virus serotype 2), which was expressed at the level of 20 mu g/L in our previous study, was higher when the expression temperature was 20 degrees C as opposed to 30 degrees C. We also tested whether the expression and functional assembly of a difficult-to-express LTB fusion protein could be increased. The assembled expression of the difficult-to-express LTB-VP1 fusion protein (LTB fused to VP1 antigen of Foot-and-Mouth Disease Virus) dramatically increased, although the total amount of expressed protein was still lower than that of LTB-EDIII2. Slight but significant increase in the expression of well-known reporter protein eGFP, which has previously been shown to be increased by cultivation at 20 degrees C, was also observed in our expression system. As no significant changes in corresponding transcripts levels and cell growth were observed between 20 degrees C and 30 degrees C, we infer that translation and post-translational assembly are responsible for these enhancements.ConclusionsThe effects of lowering the expression temperature from 30 degrees C to 20 degrees C on protein expression and folding levels in S. cerevisiae, using several proteins as models, are reported. When heterologous proteins are expressed at 20 degrees C, a greater amount of (specially, more assembled) functional proteins accumulated than at 30 degrees C. Although further studies are required to understand the molecular mechanisms, our results suggest that lowering the expression temperature is a convenient strategy for improving the expression of relatively complexly structured and difficult-to-express proteins in S. cerevisiae.

Automated summary

Lowering the expression temperature from 30 degrees C to 20 degrees C can improve the expression and functional assembly of fusion proteins in Saccharomyces cerevisiae, especially for complexly structured and difficult-to-express proteins.