Characterization of Early Gonadal Differentiation and Induction of Sex Reversal in the Rock Bream Oplegnathus fasciatus

Rock bream (Oplegnathus fasciatus) is a typical fish that has a unique multiple sex chromosome system (female X1X1X2X2/male X1X2Y). We examined the early gonadal development in rock bream via continuous histological observations of the gonads at 40-120 days post hatching (dph). The fish was identified as a typical gonochorist, and female gonads were found to differentiate earlier than male gonads. The ovarian cavity of the female was initially observed at 80 dph, whereas the efferent duct of the male was not observed until 100 dph. Immunofluorescence with the vasa-antibody revealed that germ cells were predominantly distributed around the ovarian cavity in females and on the edge of the gonad in males during the early stages of sex differentiation. Sex reversal was induced via the oral administration of letrozole (LTZ), 17 alpha-methyltestosterone (MT), and 17 beta-estradiol (E-2), respectively, during the labile period of gonadal development. LTZ and MT induced 100% masculinization of genotype-females, whereas E-2 induced only 50-60% feminization of genotype-males. Such findings suggest that the fish retained high sexual plasticity despite the existence of the neo-Y chromosome. MT and E-2 had negative effect on fish growth, whereas LTZ did not exert such side effect. LTZ and MT could accelerate gonadal development in sex-reversed genotype-males, whereas E-2 inhibited gonadal development in genotype-females of rock bream. These findings provide a basis for further research on the mechanisms of sex determination and differentiation in fishes with X1X2Y sex chromosome system and provide a sex reversal protocol for rock bream.

Automated summary

We investigated the early gonadal development in rock bream through histological observations. Females showed earlier differentiation compared to males. Treatment with letrozole (LTZ) and 17 alpha-methyltestosterone (MT) resulted in complete masculinization of genotype-females, while 17beta-estradiol (E-2) only partially feminized genotype-males. These findings contribute to the understanding of sex determination and differentiation in fishes with the X1X2Y sex chromosome system and provide a sex reversal protocol for rock bream.