期刊
MARINE BIOTECHNOLOGY
卷 25, 期 3, 页码 428-437出版社
SPRINGER
DOI: 10.1007/s10126-023-10216-5
关键词
miR-10a-3p; Neuropeptide Y; Pinctada fucata martensii; Nacre; Biomineralization
In this study, the precursor sequence of Pm-miR-10a-3p was obtained in the pearl oyster Pinctada fucata martensii and its expression level in the mantle tissues was detected. Pm-nAChRs alpha and Pm-NPY were identified as potential target genes of Pm-miR-10a-3p. Over-expression of Pm-miR-10a-3p downregulated the target genes and disrupted the nacre microstructure.
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression via the recognition of their target messenger RNAs. MiR-10a-3p plays an important role in the process of ossification. In this study, we obtained the precursor sequence of miR-10a-3p in the pearl oyster Pinctada fucata martensii (Pm-miR-10a-3p) and verified its sequence by miR-RACE technology, and detected its expression level in the mantle tissues of the pearl oyster P. f. martensii. Pm-nAChRs alpha and Pm-NPY were identified as the potential target genes of Pm-miR-10a-3p. After the over-expression of Pm-miR-10a-3p, the target genes Pm-nAChRs alpha and Pm-NPY were downregulated, and the nacre microstructure became disordered. The Pm-miR-10a-3p mimic obviously inhibited the luciferase activity of the 3 ' untranslated region of the Pm-NPY gene. When the interaction site was mutated, the inhibitory effect disappeared. Our results suggested that Pm-miR-10a-3p participates in nacre formation in P. f. martensii by targeting Pm-NPY. This study can expand our understanding of the mechanism of biomineralization in pearl oysters.
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