Feeding Strategies and Trophic Niche Divergence of Three Groups of Dosidicus gigas off Peru: Based on Stable Carbon and Nitrogen Isotopes and Morphology of Feeding Apparatuses

Dosidicus gigas (D. gigas) is a pelagic cephalopod of ecological and economic importance widely distributed in the eastern Pacific Ocean. Generally, small-, medium-, and large-sized groups of the squids have been respectively identified on the basis of the mantle length (ML) of adults. Intraspecific feeding variability maximizes the utilization of available food resources by D. gigas. However, the coexistence mechanism of three groups has not been fully understood yet. In our study, based on the analyses of beak morphology and stable carbon and nitrogen isotopes, the feeding strategies and coexistence patterns of large-, medium-, and small-sized groups of D. gigas were investigated. D. gigas had a wide range of C-13/C-12 (delta C-13) and N-15/N-14 (delta N-15) values in muscle tissue, variable feeding behaviors, and wide food sources. The delta C-13 or delta N-15 values showed no significant difference between the small- and medium-sized groups, which shared the same habitats and fed on preys with the similar trophic level. Compared to small- and medium-sized groups, the large-sized group had a smaller range of habitats and consumed more nearshore foods. Both isotopes and feeding apparatus morphology indicated a high degree of niche overlap between the small- and medium-sized groups, whereas the large-sized group differed significantly from other groups. In addition, the niche width of the female was larger than that of the male in all three groups. We inferred that the sex differences in body length and reproductive behavior led to the difference in niche width. The isotopic niche overlap between female and male samples was the most significant in large-sized group and the least significant in the small-sized group, indicating that different feeding strategies were adopted by the three groups. These findings proved that the three groups of D. gigas off Peruvian waters adopted a feeding strategy with inter- and intra-group regulation. This feeding strategy maximizes the use of food and habitat resources and ensures that different size groups can coexist in the same waters.

Automated summary

In this study, the feeding strategies and coexistence patterns of large-, medium-, and small-sized groups of D. gigas were investigated using beak morphology and stable carbon and nitrogen isotopes analysis. The results showed high niche overlap between medium- and small-sized groups, whereas the large-sized group differed significantly. Additionally, the niche width of females was larger than that of males in all groups. These findings demonstrated that the three different size groups of D. gigas adopted a feeding strategy with inter- and intra-group regulation to maximize resource utilization and enable coexistence.