期刊
MACROMOLECULAR BIOSCIENCE
卷 23, 期 7, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.202300019
关键词
biomimetic materials; gelatin; GelMA; Myoblasts; piezoionic
In this study, Gelatin Methacryloyl (GelMA) is modified with hydrophilic 2-acryloxyethyltrimethylammonium chloride (AETA) and 3-sulfopropyl acrylate potassium (SPA) ionic comonomers to create a piezoionic hydrogel. The modified GelMA exhibits increased ionic conductivity and electrical response to mechanical stress, making it suitable for tissue engineering of skeletal muscles. The viability of murine myoblasts on the piezoionic hydrogels confirms their biocompatibility and the potential for utilizing piezo-effects in tissue engineering.
For tissue engineering of skeletal muscles, there is a need for biomaterials which do not only allow cell attachment, proliferation, and differentiation, but also support the physiological conditions of the tissue. Next to the chemical nature and structure of the biomaterial, its response to the application of biophysical stimuli, such as mechanical deformation or application of electrical pulses, can impact in vitro tissue culture. In this study, gelatin methacryloyl (GelMA) is modified with hydrophilic 2-acryloxyethyltrimethylammonium chloride (AETA) and 3-sulfopropyl acrylate potassium (SPA) ionic comonomers to obtain a piezoionic hydrogel. Rheology, mass swelling, gel fraction, and mechanical characteristics are determined. The piezoionic properties of the SPA and AETA-modified GelMA are confirmed by a significant increase in ionic conductivity and an electrical response as a function of mechanical stress. Murine myoblasts display a viability of >95% after 1 week on the piezoionic hydrogels, confirming their biocompatibility. The GelMA modifications do not influence the fusion capacity of the seeded myoblasts or myotube width after myotube formation. These results describe a novel functionalization providing new possibilities to exploit piezo-effects in the tissue engineering field.
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