4.5 Article

Laccase-luminol chemiluminescence system: an investigation of substrate inhibition

期刊

LUMINESCENCE
卷 38, 期 3, 页码 341-349

出版社

WILEY
DOI: 10.1002/bio.4460

关键词

biosensor; chemiluminescence; laccase; luminol; substrate inhibition

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This study characterized the laccase-luminol system and determined its kinetic parameters. The system can produce chemiluminescence without an intermediate molecule, but it exhibits substrate-inhibition behavior. Low concentrations of luminol can avoid this inhibition effect. In summary, the laccase-luminol system has potential applications in molecular detection and quantification.
Chemiluminescence (CL) reactions are widely used for the detection and quantification of many types of analytes. Laccase has previously been proposed in CL reactions; however, its light emission behaviour has not been characterized. This study was conducted to characterize the laccase-luminol system, determine its kinetic parameters, and analyze the effects of protein and OH- concentration on the CL signal. Laccase from Coriolopsis gallica was combined with different concentrations of luminol (125 nM to 4 mM), and the enzyme kinetics were evaluated using diverse kinetic models. The laccase-luminol system was able to produce CL without an intermediate molecule, but it exhibited substrate-inhibition behaviour. A two-site random model was used and suggested that when the first luminol molecule was bound to the active site, laccase affinity for the second luminol molecule was increased. This inhibition effect could be avoided using a low luminol concentration. At 5 mu M luminol concentration, 1 mg/ml (0.13 U) laccase is needed to achieve nearly 90% of the maximum CL signal, suggesting that the available luminol could not bind to all active sites. Furthermore, the concentration of NaOH negatively affected the CL signal. The laccase-luminol system represents an alternative to existing CL systems, with potential uses in molecular detection and quantification.

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