期刊
LUMINESCENCE
卷 38, 期 3, 页码 334-340出版社
WILEY
DOI: 10.1002/bio.4457
关键词
electrochemiluminescence; Helicobacter pylori; rolling circle amplification
In this study, an electrochemiluminescence-based platform was proposed for sensitive detection of H. pylori DNA. The strategy demonstrated excellent stability and selectivity, with a low detection limit of 10 pM. Additionally, a biosensing chip was designed to improve throughput and enable large-scale clinical biosensing applications.
Helicobacter pylori is closely linked to many gastric diseases such as gastric ulcers and duodenal ulcers. Therefore, biosensing H. pylori has attracted wide attention from both scientists and clinicians. Here, we proposed an electrochemiluminescence (ECL)-based platform that could sensitively detect H. pylori DNA. In this platform, a novel target-cycling synchronized rolling circle amplification was used for signal amplification. Silver nanoclusters (Ag NCs) were synthesized on the circle DNA products, embedding them with the ability to catalyze the electrochemical reduction of K2S2O8, in turn resulting in rapid consumption of the ECL co-reactant near the working electrode, and leading to a decrease in the ECL emission intensity. In addition to its excellent stability and selectivity, the proposed strategy had a low detection limit of 10 pM, an indication that it can be beneficially applied to test biosamples. Furthermore, a biosensing chip was designed to improve the throughput and shed new light on large-scale clinical biosensing applications.
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