Polymerase chain reaction in the diagnosis of invasive aspergillosis: approaches for appropriate use

Detection of aspergillus by PCR is a helpful tool for early diagnosis. The test has excellent sensitivity and specificity with a high negative predictive value. Well-accepted, standardized method for DNA extraction for PCR testing is to be adopted for all commercial assays and conclusive validation data are awaited in varied clinical settings. This perspective offers guidance for utilizing PCR testing while awaiting such data. Quantification by PCR, species-specific identification assays and detection of resistance genetic markers are of future promise. Herein, we summarize the available data on aspergillus PCR and describe its potential utility through a clinical case scenario-based approach.

Automated summary

Detection of aspergillus by PCR is a valuable tool for early diagnosis, with high sensitivity, specificity, and negative predictive value. Standardized DNA extraction methods are recommended for all commercial assays, while awaiting conclusive validation data in different clinical settings. This perspective provides guidance for PCR testing and highlights future prospects such as quantification, species-specific identification, and detection of resistance genetic markers.