4.6 Article

Specific Residues in the C-Terminal Domain of the Human Metapneumovirus Phosphoprotein Are Indispensable for Formation of Viral Replication Centers and Regulation of the Function of the Viral Polymerase Complex

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemistry & Molecular Biology

Structural modeling, energetic analysis and molecular design of a n-stacking system at the complex interface of pediatric respiratory syncytial virus nucleocapsid with the C-terminal peptide of phosphoprotein

Haiyan Liu et al.

Summary: Respiratory syncytial virus is the main cause of respiratory tract infections in infants and children. The interaction between the virus's phosphoprotein and nucleoprotein is essential for viral replication. It has been discovered that the phenylalanine residue in the phosphoprotein plays a critical role in this interaction.

BIOPHYSICAL CHEMISTRY (2023)

Article Virology

Sequential Phosphorylation of Hepatitis C Virus NS5A Protein Requires the ATP-Binding Domain of NS3 Helicase

Chun-Chiao Yu et al.

Summary: In this study, we discovered for the first time that the ATP-binding domain of NS3 is involved in NS5A phosphorylation. In vitro assays showed that casein kinase I alpha is a very potent kinase responsible for NS5A phosphorylation at serines 225, 232, and 235. Our data suggest that ATP binding by NS3 probably results in conformational changes that recruit casein kinase I alpha to phosphorylate NS5A, initially at S225 and subsequently at S232 and S235. Our discovery reveals intricate requirements of the structural integrity of NS3 for NS5A hyperphosphorylation and HCV replication.

JOURNAL OF VIROLOGY (2022)

Article Microbiology

Human Metapneumovirus Phosphoprotein Independently Drives Phase Separation and Recruits Nucleoprotein to Liquid-Like Bodies

Kerri Beth Boggs et al.

Summary: Human metapneumovirus (HMPV) is a major cause of respiratory diseases in children, immunocompromised individuals, and the elderly. There is currently no effective treatment available. HMPV inclusion bodies (IBs) are dynamic structures required for viral replication and transcription. Recent research has suggested that HMPV IBs possess liquid organelle properties and that the viral P protein may play a role in their formation.
Article Virology

Liquid Phase Partitioning in Virus Replication: Observations and Opportunities

Chao Wu et al.

Summary: This review describes the application of phase separation in the formation of viral replication centers, with a focus on nonsegmented negative-strand RNA viruses. It discusses the interplay between phase separation and viral transcription, genome replication, and the role of these regions in pathogen-host interactions. The article also highlights the critical questions that need to be addressed to fully understand the impact of phase separation on viral replication and the viral life cycle, as well as new approaches that could lead to important breakthroughs in this emerging field.

ANNUAL REVIEW OF VIROLOGY (2022)

Review Biochemistry & Molecular Biology

Pneumoviral Phosphoprotein, a Multidomain Adaptor-Like Protein of Apparent Low Structural Complexity and High Conformational Versatility

Christophe Cardone et al.

Summary: Mononegavirales phosphoproteins P serve as essential co-factors of viral polymerase, with highly diverged but structurally conserved features. These multidomain proteins possess oligomerization domains and large intrinsically disordered regions. Through interaction with various proteins, they play a crucial role in regulating viral transcription and replication, showcasing the structural versatility of non-globular intrinsically disordered protein domains.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Review Virology

Interactions between the Nucleoprotein and the Phosphoprotein of Pneumoviruses: Structural Insight for Rational Design of Antivirals

Hortense Decool et al.

Summary: Pneumoviruses, including pathogenic human and animal viruses such as hRSV and hMPV, are major causes of severe respiratory illnesses in young children and vulnerable populations. Transcription and replication of these viruses rely on specific cytoplasmic inclusions and interactions between viral polymerase and cofactors. Recent structural studies on viral proteins aim to aid in the development of new antivirals against these viruses.

VIRUSES-BASEL (2021)

Review Public, Environmental & Occupational Health

Global burden of acute lower respiratory infection associated with human parainfluenza virus in children younger than 5 years for 2018: a systematic review and meta-analysis

Xin Wang et al.

Summary: This study estimated the burden of human parainfluenza virus (hPIV) in childhood acute lower respiratory infections (ALRI) globally, finding that approximately 13% of ALRI cases, 4-14% of ALRI hospital admissions, and 4% of childhood ALRI mortality were attributable to hPIV. The study revealed that infants aged 0-5 months and 6-11 months had the highest hospital admission and in-hospital death rates. Overall, these numbers indicate a potentially significant burden of hPIV in morbidity and mortality of ALRI in young children.

LANCET GLOBAL HEALTH (2021)

Review Cell Biology

New Perspectives on the Biogenesis of Viral Inclusion Bodies in Negative-Sense RNA Virus Infections

Olga Dolnik et al.

Summary: Infections by NSVs lead to the formation of viral inclusion bodies with properties of biomolecular condensates, facilitating efficient viral replication.
Article Multidisciplinary Sciences

Structure of the human metapneumovirus polymerase phosphoprotein complex

Junhua Pan et al.

NATURE (2020)

Review Biochemistry & Molecular Biology

Negri bodies and other virus membrane-less replication compartments

Quentin Nevers et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2020)

Review Biochemistry & Molecular Biology

Fuzzy protein theory for disordered proteins

Monika Fuxreiter

BIOCHEMICAL SOCIETY TRANSACTIONS (2020)

Review Biochemistry & Molecular Biology

The molecular language of membraneless organelles

Edward Gomes et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2019)

Article Multidisciplinary Sciences

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

K. Martin Chow et al.

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (2019)

Review Virology

To assemble or not to assemble: The changing rules of pneumovirus transmission

Nicolas Cifuentes-Munoz et al.

VIRUS RESEARCH (2019)

Article Virology

Measles Virus Forms Inclusion Bodies with Properties of Liquid Organelles

Yuqin Zhou et al.

JOURNAL OF VIROLOGY (2019)

Article Biochemistry & Molecular Biology

Biochemical characterization of the respiratory syncytial virus N0-P complex in solution

Camille Esneau et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2019)

Article Multidisciplinary Sciences

Negri bodies are viral factories with properties of liquid organelles

Jovan Nikolic et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

Structural dissection of human metapneumovirus phosphoprotein using small angle x-ray scattering

Max Renner et al.

SCIENTIFIC REPORTS (2017)

Article Virology

Taxonomy of the order Mononegavirales: update 2016

Claudio L. Afonso et al.

ARCHIVES OF VIROLOGY (2016)

Review Microbiology

Human Metapneumovirus: Lessons Learned over the First Decade

Verena Schildgen et al.

CLINICAL MICROBIOLOGY REVIEWS (2011)

Article Microbiology

Phosphorylation of paramyxovirus phosphoprotein and its role in viral gene expression

Sandra M. Fuentes et al.

FUTURE MICROBIOLOGY (2010)

Article Biotechnology & Applied Microbiology

Human metapneumovirus nucleoprotein and phosphoprotein interact and provide the minimal requirements for inclusion body formation

Aaron Derdowski et al.

JOURNAL OF GENERAL VIROLOGY (2008)

Article Biochemistry & Molecular Biology

The importance of intrinsic disorder for protein phosphorylation

LM Iakoucheva et al.

NUCLEIC ACIDS RESEARCH (2004)