Fatal balamuthosis in a Siberian tiger and a literature review of detection options for free-living amoebic infections in animals

Free-living amoebae are rare causes of morbidity and mortality in humans and animals around the globe. Because the route of exposure and clinical progression of disease caused by different species of amoebae may vary in people and animals, determining the species of amoeba present is important. We describe here a fatal infection by the free-living amoeba Balamuthia mandrillaris in a Siberian tiger (Panthera tigris altaica). The 17-y-old patient had a rapid clinical decline after a peracute onset of severe lethargy, dull mentation, and anorexia. Autopsy did not identify a cause of death. Histology revealed inflammation associated with amoebic trophozoites in the brain, lungs, and iris of one eye. These amoebae were confirmed to be B. mandrillaris based on a PCR assay and sequencing. Although there are subtle morphologic differences between cyst stages of Acanthamoeba spp., B. mandrillaris, and Naegleria fowleri when present and identified on routine staining, other modalities, including PCR, immunofluorescence, electron microscopy, and immunohistochemistry, are typically utilized to confirm the pathogen involved in these cases. We review the reports of balamuthosis in animals.

Automated summary

Free-living amoebae can cause rare diseases and death in humans and animals worldwide. Identifying the species of amoeba involved is important due to variations in exposure routes and clinical progression. This study reports a fatal infection by the free-living amoeba Balamuthia mandrillaris in a Siberian tiger, confirmed through PCR assay and sequencing. Different modalities, including PCR, immunofluorescence, electron microscopy, and immunohistochemistry, are often used to confirm the pathogen in these cases.